轉(zhuǎn)cryia基因抗蟲棉花棉籽的誘變活性研究報告.doc
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轉(zhuǎn)cryia基因抗蟲棉花棉籽的誘變活性研究報告,轉(zhuǎn)cryia基因抗蟲棉花棉籽的誘變活性研究報告本文共10頁，7200余字；摘要：分別以環(huán)磷酰胺和氟化鈉作為陽性誘變劑，以普通棉的棉籽粉及棉籽油作為對照物，同時設(shè)陰性對照組，以成年封閉群昆明種雄性小鼠和斑馬魚(branchynanio rerio)為實驗材料，將棉籽粉或棉籽油摻入飼料中飼喂小鼠或斑馬魚，檢測小鼠骨髓嗜多染...
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轉(zhuǎn)CryIA基因抗蟲棉花棉籽的誘變活性研究報告
本文共10頁，7200余字；
摘 要：分別以環(huán)磷酰胺和氟化鈉作為陽性誘變劑，以普通棉的棉籽粉及棉籽油作為對照物，同時設(shè)陰性對照組，以成年封閉群昆明種雄性小鼠和斑馬魚(Branchynanio rerio)為實驗材料，將棉籽粉或棉籽油摻入飼料中飼喂小鼠或斑馬魚，檢測小鼠骨髓嗜多染紅細(xì)胞（Polychromatic erythrocytes, PCE）微核率（Micronucleus frequency, MN‰）和精子畸變率（Sperm malformation rate），以及斑馬魚外周血紅細(xì)胞（Peripheral erythrocytes）微核率。結(jié)果表明：在本實驗條件下，轉(zhuǎn)CryIA基因抗蟲棉與普通棉的棉籽粉及棉籽粉油對小鼠體細(xì)胞和性細(xì)胞均未顯示出誘變活性，且沒有劑量效應(yīng)。腹腔注射氟化鈉可以誘導(dǎo)斑馬魚外周血紅細(xì)胞微核率的顯著升高；轉(zhuǎn)CryIA基因抗蟲棉棉籽粉粉和普通棉棉籽粉粉一樣，對斑馬魚外周血紅細(xì)胞微核率無明顯影響。魚類飼料中添加常規(guī)量的轉(zhuǎn)CryIA基因抗蟲棉棉籽粉不會誘發(fā)微核產(chǎn)生。
關(guān)鍵詞：Bt抗蟲棉,小鼠,斑馬魚,微核率,精子畸變率
Study on Mutagenicity of Cotton Seed Powder and Cotton Seed Oil
of Transgenic CryIA Cotton*
Zhang Ning，Chen Mingnan，Ma Jixia，Li Songgang，Xu Chongren**
（Department of Environmental Biology and Ecology, College of Life Sciences，
Peking University，100871）
Abstract：The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish’s diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafish (Branchynanio rerio) were assayed in this study. The sperm malformation rate in mice was also investigated. The results showed that, the seed powder and seed oil of transgenic CryIA cotton lines and regular cotton showed no cytomutagenicity or genomutagenicity on mice.
目錄：
1 材料和方法
1.1 材料
1.1.1 實驗動物
1.1.2實驗材料
1.2 方法
1.2.1 實驗分組與處理
1.2.1.1 小鼠實驗
1.2.1.2 斑馬魚實驗
1.2.2 小鼠微核試驗方法
1.2.3 小鼠精子畸形試驗
1.2.5 斑馬魚微核試驗方法
2結(jié)果
2.1 小鼠微核試驗
2.2 小鼠精子畸形試驗
2.3 斑馬魚實驗
3討論
參 考 文 獻(xiàn)
1 李復(fù)興、李希沛主編.配合飼料大全，青島海洋大學(xué)出版社，1994，p222
2 龍繁新等.B.t.i.對脊椎動物的安全實驗.蒲蟄龍主編.蘇云金桿菌以色列變種防
治蚊幼蟲的研究，中山大學(xué)出版社，1984, p29
3 郭三堆.植物B.t.抗蟲基因工程研究進(jìn)展，中國農(nóng)業(yè)科學(xué)，1995，28（5）：8
4 Schimd，W. The Micronucleus Test for Cytogenetic Analysis. Chemical Mutagens，
A. Hollaender (Ed)，Plenum，New York，1976，Vol.14：31
5 Manna，GK. Micronucleus Test in The Peripheral Erythrocytes of The Exotic Fish. The
Nucleus，1984，Vol.27(3)：203
本文共10頁，7200余字；
摘 要：分別以環(huán)磷酰胺和氟化鈉作為陽性誘變劑，以普通棉的棉籽粉及棉籽油作為對照物，同時設(shè)陰性對照組，以成年封閉群昆明種雄性小鼠和斑馬魚(Branchynanio rerio)為實驗材料，將棉籽粉或棉籽油摻入飼料中飼喂小鼠或斑馬魚，檢測小鼠骨髓嗜多染紅細(xì)胞（Polychromatic erythrocytes, PCE）微核率（Micronucleus frequency, MN‰）和精子畸變率（Sperm malformation rate），以及斑馬魚外周血紅細(xì)胞（Peripheral erythrocytes）微核率。結(jié)果表明：在本實驗條件下，轉(zhuǎn)CryIA基因抗蟲棉與普通棉的棉籽粉及棉籽粉油對小鼠體細(xì)胞和性細(xì)胞均未顯示出誘變活性，且沒有劑量效應(yīng)。腹腔注射氟化鈉可以誘導(dǎo)斑馬魚外周血紅細(xì)胞微核率的顯著升高；轉(zhuǎn)CryIA基因抗蟲棉棉籽粉粉和普通棉棉籽粉粉一樣，對斑馬魚外周血紅細(xì)胞微核率無明顯影響。魚類飼料中添加常規(guī)量的轉(zhuǎn)CryIA基因抗蟲棉棉籽粉不會誘發(fā)微核產(chǎn)生。
關(guān)鍵詞：Bt抗蟲棉,小鼠,斑馬魚,微核率,精子畸變率
Study on Mutagenicity of Cotton Seed Powder and Cotton Seed Oil
of Transgenic CryIA Cotton*
Zhang Ning，Chen Mingnan，Ma Jixia，Li Songgang，Xu Chongren**
（Department of Environmental Biology and Ecology, College of Life Sciences，
Peking University，100871）
Abstract：The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish’s diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafish (Branchynanio rerio) were assayed in this study. The sperm malformation rate in mice was also investigated. The results showed that, the seed powder and seed oil of transgenic CryIA cotton lines and regular cotton showed no cytomutagenicity or genomutagenicity on mice.
目錄：
1 材料和方法
1.1 材料
1.1.1 實驗動物
1.1.2實驗材料
1.2 方法
1.2.1 實驗分組與處理
1.2.1.1 小鼠實驗
1.2.1.2 斑馬魚實驗
1.2.2 小鼠微核試驗方法
1.2.3 小鼠精子畸形試驗
1.2.5 斑馬魚微核試驗方法
2結(jié)果
2.1 小鼠微核試驗
2.2 小鼠精子畸形試驗
2.3 斑馬魚實驗
3討論
參 考 文 獻(xiàn)
1 李復(fù)興、李希沛主編.配合飼料大全，青島海洋大學(xué)出版社，1994，p222
2 龍繁新等.B.t.i.對脊椎動物的安全實驗.蒲蟄龍主編.蘇云金桿菌以色列變種防
治蚊幼蟲的研究，中山大學(xué)出版社，1984, p29
3 郭三堆.植物B.t.抗蟲基因工程研究進(jìn)展，中國農(nóng)業(yè)科學(xué)，1995，28（5）：8
4 Schimd，W. The Micronucleus Test for Cytogenetic Analysis. Chemical Mutagens，
A. Hollaender (Ed)，Plenum，New York，1976，Vol.14：31
5 Manna，GK. Micronucleus Test in The Peripheral Erythrocytes of The Exotic Fish. The
Nucleus，1984，Vol.27(3)：203
