摘  要
　　　大豆異黃酮是從大豆中提取出來(lái)的一類微量活性物質(zhì)，具有多種重要的生理功能。本論文以豆渣為實(shí)驗(yàn)材料，研究了大豆異黃酮的提取工藝。
　　　有機(jī)溶劑萃取法或浸泡提取法是提取大豆異黃酮的一種常用的提取方法。本實(shí)驗(yàn)以乙醇為溶劑，大豆豆渣為原料，研究了大豆異黃酮的提取工藝，選取了濃度、提取時(shí)間、液料比、溫度四個(gè)變量對(duì)大豆異黃酮提取工藝的影響，并進(jìn)行了響應(yīng)曲面法優(yōu)化分析，建立了相關(guān)數(shù)學(xué)模型。研究結(jié)果表明，乙醇濃度、溫度、提取時(shí)間、液料比對(duì)豆渣中大豆異黃酮的提取均產(chǎn)生不同程度的影響。從實(shí)驗(yàn)結(jié)果得知，乙醇濃度對(duì)豆渣異黃酮提取量的影響最大，提取時(shí)間對(duì)大豆異黃酮提取量的影響最小。通過(guò)響應(yīng)曲面法得出大豆異黃酮提取的最佳工藝條件，乙醇濃度70%、溫度60℃、提取時(shí)間5h、液料比16mL/g。此時(shí)提取量可達(dá)7.72mg/10g，與實(shí)驗(yàn)值7.68mg/10g基本吻合。因此，該實(shí)驗(yàn)方案具有一定的可行性，該模型具有一定的理論價(jià)值，可為相關(guān)工業(yè)生產(chǎn)提供一定的理論依據(jù)。
　　　
關(guān)鍵詞：大豆異黃酮  豆渣  提取率  響應(yīng)曲面法優(yōu)化
　　　

　　Optimization of isoflavones extraction from soybean dregs using response surface methodology
ABSTRACT
　　　　
　　　Soybean isoflavones from soybean extracted trace active substances,has many important physiological functions.Bean dregs as experimental material,this paper studied the soybean isoflavone extraction technology.
　　　Organic solvent extraction or soaking extraction is a king of commonly used to extract soybean isoflavone extraction method.This experiment with ethanol as solvent,soy bean dregs as raw material,soybean isoflavone extraction technology was studied and selected the concentration,extraction time and solid-liquid ratio,temperature four variables impact on soybean isoflavone extraction technology,and has carried on the response surface method optimization analysis,the relevant mathematical model is established.The research results show that ethanol concentration,temperature,extraction time and solid-liquid ratio on bean dregs in the extraction of soybean isoflavones are varying degress of impact.From the experiment results indicate that the ethanol concentration affects the quantity of bean dregs isoflavone extraction,the largest amount of time for extracting soybean isoflavines minimal impact. By response surface method for optimum process condition of soybean isoflavones extract,ethanod concentration 70%,5h temperature 60℃,  extraction time, liquid than 16 ml/g. Extract the quantity at this time can be up to 7.72 mg/g, with the experimental value of 7.68 mg / 10 g. This experiment scheme has certain feasibility, therefore, has certain theory value, the model can provide certain theoretical basis for related industrial production.
　　　                 
　　　Keywords: soybean isoflavone; bean dregs; extraction yield; response surface methodoloy 

目  錄
摘  要 I
Abstract II
目  錄 III
1.緒  論 1
1.1大豆異黃酮研究概況 1
1.1.1大豆異黃酮的簡(jiǎn)要概述 1
1.1.2大豆異黃酮的成分及分布 1
1.1.3豆渣異黃酮測(cè)定方法 3
1.1.4豆渣異黃酮提取工藝的研究狀況 4
1.2選題意義及研究?jī)?nèi)容 5
2.實(shí)驗(yàn)部分 6
2.1實(shí)驗(yàn)儀器、材料和試劑 6
2.1.1實(shí)驗(yàn)儀器 6
2.1.2實(shí)驗(yàn)材料 6
2.1.3 實(shí)驗(yàn)試劑 6
2.2實(shí)驗(yàn)內(nèi)容 7
2.2.1 豆渣異黃酮提取工藝流程 7
2.3實(shí)驗(yàn)方法 7
2.3.1原料預(yù)處理 7
2.3.2豆渣脫脂 7
2.3.3干燥 8
2.3.3加熱浸提 8
2.3.4料液過(guò)濾 8
2.3.5異黃酮的含量測(cè)定方法 8
3．結(jié)果與分析 10
3.1單因素試驗(yàn)法 10
3.1.1乙醇濃度對(duì)大豆異黃酮提取的影響 10
3.1.2溫度對(duì)大豆異黃酮提取量的影響 11
3.1.3提取時(shí)間對(duì)大豆異黃酮提取量的影響 11
3.1.4料液比對(duì)大豆異黃酮提取量的影響 12
3.2豆渣異黃酮的響應(yīng)曲面優(yōu)化法 13
3.2.1中心組合(CCD)試驗(yàn)設(shè)計(jì) 13
3.2.2模型的建立及其顯著性檢驗(yàn) 15
3.2.3豆渣異黃酮提取的響應(yīng)曲面分析與優(yōu)化 16
結(jié)  論 21
參考文獻(xiàn) 22
致  謝 23