GSH對低溫脅迫下枇杷幼果葉綠體AsA-GSH循環(huán)代謝的影響

目 錄

1 引言 2
2 材料與方法 2
2.1 供試材料 2
2.2 主要試劑與儀器 2
2.3 測定項目與方法 2
2.4 數(shù)據(jù)分析 3
3 結(jié)果與分析 3
3.1 GSH對低溫脅迫下枇杷幼果葉綠體GSH含量的影響 3
3.2 GSH對低溫脅迫下枇杷幼果葉綠體AsA含量的影響 3
3.3 GSH對低溫脅迫下枇杷幼果葉綠體中MDHAR活性的影響 3
4 討論 3
5 結(jié)論 4
致謝 4
參考文獻 4
附錄 5
　
　


摘要：探討GSH在提高枇杷(Eriobotrya japonica Lindl.)幼果抗寒性方面的應用。以3年生“早鐘6號”枇杷(Eriobotrya japonica Lindl.cv. Zaozhong NO.6)容器苗為試材，研究不同質(zhì)量濃度谷胱甘肽(GSH)處理對﹣3℃條件下枇杷幼果葉綠體AsA-GSH循環(huán)代謝的影響及其在抗低溫脅迫中的作用。低溫脅迫下枇杷幼果葉綠體抗氧化劑還原型谷胱甘肽(GSH)和抗壞血酸(AsA)含量下降, 多種重要酶的活性受到不同程度的抑制。采用GSH處理可顯著提高低溫脅迫下枇杷幼果葉綠體GSH等的活性，促進AsA-GSH循環(huán)，在枇杷幼果抵抗低溫脅迫中發(fā)揮作用。
關鍵詞：葉綠體; 還原型谷胱甘肽(GSH); 低溫脅迫; AsA-GSH循環(huán)代謝

Effects of GSH on AsA-GSH Circulation Metabolism in Chloroplasts of Young Loquat Fruits Under Low Temperature Stress



Abstract: The paper studied the application of reduced glutathione (GSH) in increasing the chilling resistance abilities of young loquat (Eriobotrya japonica Lindl.cv. Zaozhong NO.6) fruits. The container seedling of three-year-old loquat were treated with 100, 300 and 500 mg·L-1 of GSH. The study was to investigate the effects of exogenous GSH on AsA-GSH circulation metabolism in chloroplasts of young loquat fruits under low temperature stress, and its effects on the resistance to low temperature stress. The results showed that the contents of GSH and ascorbic acid(AsA) in chloroplasts decreased while the activities of some important enzymes also decreased when the young loquat fruits were under low temperature stress. The treatment with 100 mg·L-1 of GSH on young loquat fruits could significantly improve the GSH, AsA contents and APX, GR, MDHAR activities. Furthermore, the treatment promoted AsA-GSH circulation metabolism, and improved the ability of free radicals to scavenge in chloroplasts. The results demonstrated that exogenous GSH played a role in the low temperature stress resistance of young loquat fruits.
Keywords: chloroplast; reduced glutathione (GSH); low temperature strees; AsA-GSH circulation metabolism