結(jié)石通片中綠原酸的含量測(cè)定

摘要：目的：建立HPLC法測(cè)定結(jié)石通片中綠原酸含量的方法。方法：采用高效液相法，色譜柱為 sinochrom ODS-BP(4.6mm ×250mm 5μm),以乙腈：0.5%磷酸溶液（8:92）為流動(dòng)相，檢測(cè)波長(zhǎng)為326nm，流速為1.0ml/min。結(jié)果：綠原酸在濃度為0.008125~0.13mg/ml呈線性關(guān)系r=0.9997（n=6),平均回收率為99.46%，RSD=0.82%。結(jié)論：本法簡(jiǎn)便、準(zhǔn)確、快速，適用于結(jié)石通片中綠原酸的含量測(cè)定。
關(guān)鍵詞：高效液相法 結(jié)石通片 綠原酸 含量測(cè)定

Determination of Chlorogenic Acid in Jieshitong pian by HPLC

Abstract:Objective: Establish a High-performance liquid chromatography (HPLC) to determining of Chlorogenic acid in Jieshitong pian.Methods: Using high performance liquid chromatography, the determination was performed on sinochrom ODS-BP(4.6mm ×250mm 5μm) column,mobile phase for acetonitril- e-0.5% phosphoric acid solution(8:92), detected wavelength for 326 nm, velocity of 1.0 mL/min.Results: The linear range of Chlorogenic acid was 0.4024 -3.2192 u g/ml (r = 0.9997 n = 6),the average recovery is 99.46%, and the RSD (n = 9) is 0.82%. Conclusion: The method was proved to be simple,,reproducible and specific, and it’s propitious to used for determining of Chlorogenic acid in Jieshitong pian.
Keywords: HPLC Jieshitong pian Chlorogenic acid Content determination