摘  要：兩親性共聚物以其優(yōu)越的性能開(kāi)始備受矚目，采用聚對(duì)苯二甲酸乙二醇酯(PET)為基材制備出兩親性的薄膜表面具有廣泛的開(kāi)發(fā)應(yīng)用前景。本論文采用分子設(shè)計(jì)的方法，以聚乙二醇單甲醚(mPEG)和丙烯酰氯為原料，通過(guò)酯化反應(yīng)對(duì)mPEG進(jìn)行末端功能化修飾；在PET薄膜表面引發(fā)原子轉(zhuǎn)移自由聚合接枝mPEG衍生物與苯乙烯的共聚物，得到具有兩親性表面的功能化PET薄膜。研究聚合時(shí)間以及共聚單體比例對(duì)接枝效果及兩親性能的影響。利用衰減全反射傅立葉變換紅外光譜儀(FTIR/ATR)、X-射線光電子能譜(XPS)、掃描電子顯微鏡(SEM)和接觸角測(cè)試儀(CA)對(duì)修飾后聚酯薄膜進(jìn)行表征。采用大腸桿菌(E.coli)粘附實(shí)驗(yàn)考察改性前后PET薄膜表面的微生物的粘附性關(guān)系。測(cè)試結(jié)果表明：成功制備mPEG末端功能化單體，SI-ATRP方法在PET薄膜表面接枝上了兩親性mPEG衍生物與苯乙烯的共聚物層，且隨著單體比例的不同共聚物層的組成及性能均發(fā)生改變。E.coli粘附實(shí)驗(yàn)表明：隨著膜表面共聚物層中mPEG含量的增加，E.coli的粘附量減少。

關(guān)鍵字：聚酯薄膜；表面引發(fā)原子轉(zhuǎn)移自由基聚合；兩親性；微生物粘附 

Preparation of Polyester Films with Apamphipathilic Surface

Abstract: Amphiphilic copolymer with its superior performance has attracted more and more attention. Using polyethylene terephthalate (PET) as the substrate to prepare film with amphiphilic surface has wide development and application prospect. In this thesis, molecular design methods was adapted, the terminal function of polyethylene glycol monomethyl ether (mPEG) was modified through the way of esterification with polyethylene glycol monomethyl ether and acryloyl chloride as raw materials. And then the copolymer of mPEG derivatives and styrene was grafted from the surface of polyester films through surface initiated atom transfer radical polymerization (SI-ATRP) to prepare functional PET films with amphipathy surface. Research the effects of polymerization time and different feed ratio of mPEG derivatives and styrene on the grafting  effects and amphiphilic properties. The modified PET films were characterized by attenuated total reflection Fourier transform infrared spectroscopy (FTIR/ATR), X-ray photoelectron spectroscopy (XPS) , scanning electron microscopy (SEM) and contact angle(CA). The microbial adhesion of PET film before and after modification was investigated by Escherichia coli (E.coli) adhesion experiment. Test results showed: the terminal function of mPEG was successfully modified; the amphiphilic mPEG derivatives with styrene copolymer layer was grafted onto the surface of the PET film by SI-ATRP , and the composition and properties of the copolymer layer changed with the monomer ratio. The E.coli adhesion experiments showed: As the mPEG content in the copolymer layer on the film surface increases, the adhesion of E.coli reduced.

Key words: polyester films; surface-initiated atom transfer radical polymerization; apamphipathilic; microbial adhesion

目  錄

摘  要 I

Abstract II

目  錄 III

術(shù) 語(yǔ) 表 V

1 緒論 1

1.1 兩親性共聚物發(fā)展現(xiàn)狀 1

1.2 聚乙二醇單甲醚(mPEG) 1

1.3 聚酯薄膜的現(xiàn)狀和發(fā)展趨勢(shì)及其接枝改性的研究現(xiàn)狀 1

1.4 兩親性聚酯薄膜在生物醫(yī)學(xué)領(lǐng)域發(fā)展趨勢(shì) 2

1.5 論文研究意義 2

2 實(shí)驗(yàn)部分 4

2.1 原料和試劑 4

2.2 主要實(shí)驗(yàn)儀器和設(shè)備 5

2.3 原料的合成及純化 5

2.4 PET薄膜表面ATRP引發(fā)劑的固定 6

2.5 mPEG衍生物的制備 6

2.6 PET薄膜表面接枝mPEG衍生物和苯乙烯共聚物 7

2.7 細(xì)菌粘附實(shí)驗(yàn) 7

2.7.1 細(xì)菌的復(fù)蘇、培養(yǎng) 7

2.7.2 細(xì)菌生長(zhǎng)曲線的測(cè)定與比濁法 8

2.7.3 細(xì)菌粘附實(shí)驗(yàn)操作流程 8

2.8 表征 9

3 結(jié)果與討論 11

3.1 PET薄膜引發(fā)劑的X-射線光電子能譜表征 11

3.2 mPEG衍生物的表征 12

3.2.1 mPEG衍生物的紅外表征 12

3.2.2 mPEG衍生物的核磁表征 13

3.3 PET薄膜表面接枝兩親性mPEG衍生物與苯乙烯共聚物的表征 13

3.3.1 PET薄膜表面接枝兩親性mPEG衍生物與苯乙烯共聚物的紅外表征 13

3.3.2 PET薄膜表面接枝mPEG衍生物/苯乙烯共聚物的接枝量計(jì)算與分析 14

3.3.3 PET薄膜表面接枝mPEG衍生物/苯乙烯共聚物的接觸角分析 15

3.3.4 PET薄膜表面接枝mPEG衍生物/苯乙烯共聚物的表面自由能的計(jì)算及結(jié)果分析 18

3.3.5 PET薄膜表面接枝mPEG衍生物/St共聚物的表面形貌 19

3.4 細(xì)菌粘附實(shí)驗(yàn)結(jié)果分析 20

3.4.1 大腸桿菌生長(zhǎng)曲線測(cè)定分析 20

3.4.2 接枝mPEG衍生物和St共聚物改性前后的PET薄膜大腸桿菌的粘附性狀況 21

3.4.3 PET薄膜的表面性能與大腸桿菌的粘附性關(guān)系 22

4 結(jié)論 24

參 考 文 獻(xiàn) 25

致 謝 27