目錄
1.論文題目、姓名、學(xué)院........................................... 2
2. 中文摘要、英文摘要...........................................2-3
3. 引言..........................................................3
4.實(shí)驗(yàn)材料與方法步驟:.............................................3
4.1實(shí)驗(yàn)材料..................................................................................... 3
4.2方法步驟：.................................................3
4.2.1材料處理...............................................3
4.2.1.1預(yù)處理...........................................3
4.2.1.2取材.............................................4
4.2.1.3處理.............................................4
4.2堿性品紅溶液染色及形態(tài)學(xué)觀測(cè)................................4
4.3細(xì)胞凋亡計(jì)數(shù)統(tǒng)計(jì)............................................4
5.結(jié)果與分析......................................................4
5.1細(xì)胞凋亡的形態(tài)學(xué)變化.....................................4-5
5.2細(xì)胞凋亡率................................................5
6.討論..........................................................5-6
7.參考文獻(xiàn)......................................................6-7
8.致謝...........................................................7





甲醛脅迫對(duì)洋蔥內(nèi)表皮細(xì)胞調(diào)往的誘導(dǎo)
付濤，生命科學(xué)學(xué)院

摘要： 細(xì)胞凋亡是當(dāng)今環(huán)境毒理學(xué)領(lǐng)域的研究熱點(diǎn)之一．為了探討甲醛能否誘導(dǎo)細(xì)胞凋亡，以洋蔥內(nèi)表皮細(xì)胞為實(shí)驗(yàn)材料，通過(guò)不同濃度的甲醛處理一段時(shí)間后，應(yīng)用形態(tài)學(xué)觀察、顯微鏡觀察實(shí)驗(yàn)檢測(cè)了洋蔥內(nèi)表皮細(xì)胞凋亡情況．實(shí)驗(yàn)結(jié)果表明低濃度甲醛 (≤0．2mmol•L)處理洋蔥內(nèi)表皮細(xì)胞一段時(shí)間后，對(duì)細(xì)胞生長(zhǎng)有顯著的抑制作用 ，但對(duì)洋蔥內(nèi)表皮細(xì)胞形態(tài)改變不大，且對(duì)細(xì)胞核DNA沒(méi)有損傷；而高濃度甲醛 (≥0．4mmol•L)處理細(xì)胞 一段時(shí)間后，對(duì)細(xì)胞生長(zhǎng)有極顯著的抑制作用，細(xì)胞形態(tài)逐漸變的模糊不清，并有少量細(xì)胞萎縮成圓形， 且會(huì)造成細(xì)胞核DNA有規(guī)律的斷裂，細(xì)胞凋亡率隨甲醛濃度的增加而增大．以上實(shí)驗(yàn)結(jié)果顯示，高濃度甲醛處理洋蔥內(nèi)表皮細(xì)胞一段時(shí)間后，會(huì)誘導(dǎo)細(xì)胞凋亡；并且隨甲醛濃度的升高凋亡率逐漸 增大，呈一定的劑量一效應(yīng)關(guān)系．
關(guān)鍵詞：細(xì)胞凋亡；甲醛；洋蔥內(nèi)表皮細(xì)胞；顯微鏡

Abstract： Nowadays apoptosis has gained much interest in the environmentaltoxicology．In order to explore whether formaldehyde could induce apoptosis or not， Onion epidermal cells were treated with different concentrations of fomraldehyde for a period of time ．Morphologic observation、Microscopic observation of experimental were applied to detect the apoptosis of Onion epidermal cells in this experiment．Results showed that Onion epidermal cells’ prolifeation was significantly inhibited alter treated with FA atlow concentrations (≤ 0．2mmol/L )ina period of time ，while morphological change of cells was negligible，and no significant damage of cellular DNA wasobserved under the conditions．However，Onion epidermal cells’ proliferation was statistically significantly inhibited after treated with FA a thigh concentrations(≥0．4mmol/L)ina period of time ，nada small amount of cells shrank intorotundity．Meanwhile，cell nucleus DNA was broken intoregularfragment，and apoptosis rate was increased with the elevation of FA concentrations．These experiments showed hat the treatment of Onion epidermalcells by FA a thigh concentrations of r a period of time might induce cell apoptosis；andtherewasna obviously dose—depend entre lationship between FA concentrations and apoptosisrate．
Key words：apoptosis； formaldehyde；Onion epidermal cells；microscope