Effect and Mechanism of some Valid Components of Chinese Herbs in the LPS-Induced Abortion Rats
Graduate student:Wei Yanming
Supervisor:Prof. Zhao Xingxu

Pregnancy is a complex process regulated precisely at multilevels by numerous factors such as hormones, cytolcines and other molecules, however, the functions of most these factors during pregnancy has not been fully elucidated. The etiological causes of recurrent reproductive failure are diverse, many of them are not evident in routine clinical practice and some are still unknown to sophisticated researchers in the field of reproduction medicine. Generally the causes of recurrent pregnancy loss are classified as genetic, endocrinologic, anatomic, immunologic, microbiologic, and environmental. There is much evidence that cytokines play a vital role in the establishment and maintenance of pregnancy by modulating immune and endocrine systems. Evidence from many studies on marine and human pregnancy points to a strong association between maternal Th2-type immunity and successful pregnancy on the one hand and between Thl-type immune reactivity and pregnancy loss on the other. Th2-type immunity cells may play protective roles during pregnancy. Progesterone and estrogen are central to the maintenance of pregnancy, and is thus the ideal target for fertility regulation. Progesterone and estrogen have been known to exert extensive effects via progesterone receptor (PR) and estrogen receptor (ER) on diverse physiological and developmental functions of the pregnancy,meanwhile,the receptor expression patterns change dramatically during pregnancy process.
早期胚胎死亡或胚胎丟失，又稱隱性流產(chǎn)，嚴(yán)重影響母畜繁殖力與生產(chǎn)力。在人醫(yī)臨床上，自然流產(chǎn)的發(fā)生率占全部妊娠15%左右，多數(shù)為早期流產(chǎn)。妊娠時(shí)母體能夠耐受同時(shí)帶有父方遺傳基因與母方遺傳基因的半同種異體胎兒，是極其復(fù)雜而又受到精細(xì)調(diào)控的免疫學(xué)現(xiàn)象。許多證據(jù)表明Thl和Th2細(xì)胞因子在妊娠過(guò)程起著調(diào)節(jié)免疫和內(nèi)分泌的關(guān)鍵作用。雌激素和孕激素在母畜懷孕過(guò)程以至整個(gè)生殖活動(dòng)和其他生理活動(dòng)過(guò)程中均具有重要作用，雌激素受體和孕激素受體則是實(shí)現(xiàn)這些功能所必需的基本元件。
為了研究中藥或中藥成分對(duì)實(shí)驗(yàn)性流產(chǎn)大鼠保胎的作用及其機(jī)理，本實(shí)驗(yàn)采用懷孕7天大鼠尾靜脈注射細(xì)菌脂多糖，建立SD大鼠流產(chǎn)模型，再隨機(jī)分為正常對(duì)照組、流產(chǎn)模型組、黃芪多糖組、淫羊藿組和白術(shù)黃芩組。分別于懷孕4~9天口服黃芪多糖、淫羊藿提取物和白術(shù)黃芩2ml/日，對(duì)照組與模型組分別于4~9天口服生理鹽水2m1/日。于實(shí)驗(yàn)第10天麻醉處死大鼠，計(jì)數(shù)妊娠成功率和流產(chǎn)率，并以雙抗體夾心ELISA法檢測(cè)血漿和子宮組織勻漿中Thl型細(xì)胞因子IL-2, TNF-α和IFN-y,Th2型細(xì)胞因子IL-4, IL-10及子宮組織勻漿中ER, PR的含量變化，用RT-PCR法檢測(cè)子宮組織勻漿中ER mRNA和PR mRNA的表達(dá)變化，同時(shí)，用光鏡觀察子宮組織結(jié)構(gòu)的病理學(xué)變化，研究黃芪多糖、淫羊藿提取物和白術(shù)黃芩水煎液干預(yù)對(duì)妊娠結(jié)局和以上指標(biāo)的影響。本研究表明:
1 .LPS誘導(dǎo)大鼠流產(chǎn)模型流產(chǎn)率高達(dá)72.73%，對(duì)照組為8.33%，兩者差異極顯著(P<0.01)。
2.流產(chǎn)模型組大鼠血漿和子宮組織勻漿Th1型細(xì)胞因子IL-2、TNF-α和IFN-y含量顯