運用16srrna基因序列測定技術對羊胚胎胃腸 畢業(yè)論文.doc
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運用16srrna基因序列測定技術對羊胚胎胃腸 畢業(yè)論文,言···························································································································11.1 研究的目的與意義·······················...
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此文檔由會員 xiao6jun6 發(fā)布
言···························································································································1
1.1 研究的目的與意義·······························································································1
1.2宿主與胃腸道微生物的關系················································································1
1.3 16SrRNA基因檢測技術的產生···········································································2
1.4 16SrRNA基因檢測技術的應用···········································································2
2 材料與方法··············································································································3
2.1 材料·······················································································································3
2.1.1 實驗動物············································································································3
2.1.2 生化及分子生物學試劑····················································································3
2.1.3 腸道基因組DNA提取所需溶液·····································································3
2.1.4 實驗主要器材····································································································4
2.2 方法·······················································································································4
2.2.1羊胚胎胃腸道細菌分離純化·············································································4
2.2.2 分離細菌的菌落及形態(tài)特征············································································4
2.2.3 16SrRNA基因同源性分析鑒定分離細菌·························································5
2.2.3.1 分離細菌基因組DNA的提取······································································5
2.2.3.2 分離細菌16SrRNA基因的PCR擴增·························································5
2.2.3.3 PCR產物的回收與純化·················································································5
2.2.3.4 16SrDNA文庫的構建與篩選········································································6
2.2.3.5 重組陽性克隆的鑒定及測序········································································7
2.2.3.6 重組陽性克隆的酶切及RCR鑒定······························································8
2.2.3.7 16SrRNA基因序列測定及同源性分析························································8
3 結果與討論·············································································································9
3.1 結果······················································································································9
3.1.1 分離細菌DNA提取·············································································..
1.1 研究的目的與意義·······························································································1
1.2宿主與胃腸道微生物的關系················································································1
1.3 16SrRNA基因檢測技術的產生···········································································2
1.4 16SrRNA基因檢測技術的應用···········································································2
2 材料與方法··············································································································3
2.1 材料·······················································································································3
2.1.1 實驗動物············································································································3
2.1.2 生化及分子生物學試劑····················································································3
2.1.3 腸道基因組DNA提取所需溶液·····································································3
2.1.4 實驗主要器材····································································································4
2.2 方法·······················································································································4
2.2.1羊胚胎胃腸道細菌分離純化·············································································4
2.2.2 分離細菌的菌落及形態(tài)特征············································································4
2.2.3 16SrRNA基因同源性分析鑒定分離細菌·························································5
2.2.3.1 分離細菌基因組DNA的提取······································································5
2.2.3.2 分離細菌16SrRNA基因的PCR擴增·························································5
2.2.3.3 PCR產物的回收與純化·················································································5
2.2.3.4 16SrDNA文庫的構建與篩選········································································6
2.2.3.5 重組陽性克隆的鑒定及測序········································································7
2.2.3.6 重組陽性克隆的酶切及RCR鑒定······························································8
2.2.3.7 16SrRNA基因序列測定及同源性分析························································8
3 結果與討論·············································································································9
3.1 結果······················································································································9
3.1.1 分離細菌DNA提取·············································································..
